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Lecturer(s)
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Zusková Eliška, MVDr. Ph.D.
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Steinbach Christoph, Dipl.-Biol.
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Course content
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The main themes are: 1) Introduction to histology 2) Sampling and fixation methods for histology 3) Methods of drainage and saturation of samples 4) Methods for embedding in paraffin samples 5) Methods of slicing tissue blocks 6) Methods for attaching slices to slides 7) Methods of cleaning and staining sections 8) Microscopy and special histology 9) Methods of fitting colored sections 10) Processing of freshwater crayfish samples 11) Special histology of freshwater crayfish 12) Critical points of histological processing and evaluation of fish and crayfish tissues Study supporting materials at e-learning portal of USB (LMS Moodle): https://elearning.jcu.cz/course/view.php?id=2445
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Demonstration, Laboratory, E-learning, Individual tutoring, Practical training
- Preparation for classes
- 10 hours per semester
- Preparation for exam
- 20 hours per semester
- Preparation for credit
- 10 hours per semester
- Semestral paper
- 30 hours per semester
- Class attendance
- 40 hours per semester
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Learning outcomes
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The aim of the course is to acquaint students with processing and evaluation of samples - focusing on processing and evaluation of tissues closely related to their final work. The course is divided into a theoretical lectures and a practical laboratory part, where students will learn histological techniques of processing on their own samples.
Basic medical knowledge, orientation in individual organ structures, control of basic laboratory techniques and skills.
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Prerequisites
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Students are expected to have a basic knowledge of the anatomy and physiology of aquatic organisms which the student will work with. Furthermore, it is appropriate to acquire knowledge of tissue structure at the cellular level.
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Assessment methods and criteria
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Oral examination, Seminar work
Seminar work, oral exam.
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Recommended literature
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Frank Kirschbaum, Krzysztof Formicki. The Histology of Fishes. USA, 2019. ISBN 9781498784474.
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Genten, F., Terwinghe, E., Danguy, A. Atlas of Fish Histology. USA, 2008. ISBN 9781439843468.
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Takashima, F. T., Hibiy, A. An atlas of fish histology: Normal and Pathological features. Tokyo, 1995. ISBN 9783437307829.
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