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Lecturer(s)
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Steinbach Christoph, Dipl.-Biol.
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Zusková Eliška, MVDr. Ph.D.
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Course content
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Toxicity and OECD guidelines 2) Pollutants; their absorption, distribution, metabolism and excretion from fishes 3) Fundamentals in pathology and toxicity of fishes 4) Tissue-specific responses to pollution, nanotoxicology, immunotoxicology, 5) Responses of various organ systems to contaminants 6) Effects of contaminants to the behaviour of aquatic animals 7) Genotoxicity 8) Fish as a model organisms 9) Bacterial and viral fish pathology.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Demonstration, E-learning, Practical training
- Semestral paper
- 8 hours per semester
- Class attendance
- 56 hours per semester
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Learning outcomes
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Aim of the course is to give students insight into pathological effects of environmental contaminants (pesticides, pharmaceuticals, nanoparticles, organic chemicals, cyanides, alge toxins etc.) and ecological safety of these compounds. The course i) covers problems of functional changes in cells, tissues, and organs when exposed to contaminants in concentrations causing lethal and/or chronic toxicity, ii) looks at fishes to be model organisms and iii) describes the application of fish in toxicity tests specific to OECD guidelines.
The practical part will concern necropsy techniques, haematological methods and the determination and quantification of histopathological changes and cytological changes on ultrastructure level. The standardized evaluation of the histopathological changes will be practised by the determination of the tissue-specific damage index (liver, gill, skin, kidney and heart). The standardized documentation and measurement of the pathological lesion will be trained. Furthermore, the determination of teratogenic changes in fishes during their ontogenesis will be taught.
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Prerequisites
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No
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Assessment methods and criteria
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Student performance assessment, Test, Seminar work
Presence on 75% of training and lab. work, oral presentation
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Recommended literature
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Dietrich, D.R., Krieger. Histological analysis of endocrine disruptive effects in small laboratory fish. 2009.
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Giulio, R.T.D., Hinton, D.E. The Toxicology of Fishes. 2005.
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Roberts, R.J.. Fish pathology.. 2012.
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