Course: Disorders of Endocrine System in Aquatic Animals

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Course title Disorders of Endocrine System in Aquatic Animals
Course code VURH/PESD
Organizational form of instruction Lecture + Lesson + Seminary
Level of course Doctoral
Year of study not specified
Semester Winter
Number of ECTS credits 5
Language of instruction Czech
Status of course Compulsory-optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Kocour Kroupová Hana, doc. Ing. Ph.D.
  • Šauer Pavel, Ing. Ph.D.
Course content
The main topics of the course are as follows: 1) Endocrine disruption - introduction 2) Invertebrates: endocrine system, endocrine disruption 3) Fish: endocrine system, steroid hormones, endocrine disruption 4) Amphibians: endocrine system, steroid hormones, endocrine disruption, amphibians as model organisms 5) Hormonal activities - steroid receptors 6) Hormonal activities - nonsteroidal receptors 7) Natural endocrine disruptors 8) Transgenerational transmission, epigenetics 9) Gene expression analysis - introduction, reverse transcription 10) Gene expression analysis - quantitative PCR 11) In vitro tests

Learning activities and teaching methods
Dialogic (discussion, interview, brainstorming), Laboratory, Individual preparation for exam, Individual tutoring
  • Class attendance - 28 hours per semester
  • Semestral paper - 50 hours per semester
  • Preparation for classes - 70 hours per semester
Learning outcomes
The aim of the course is to describe the functioning of the endocrine system of lower aquatic vertebrates and invertebrates, various mechanisms of action of major groups of endocrine disruptors, consequences of disruption and its manifestations. Transgeneration transfer and epigenetics of these disorders will be mentioned. Methods for monitoring the effect of endocrine disruptors on aquatic organisms (in vitro and in vivo assays, gene expression analysis) will also be described. The course will include a short lab-practice, where students will be acquainted with selected methods.
Students will learn about disorders of the reproductive system of aquatic organisms caused by substances with (anti-)estrogenic and (anti-)androgenic effects, which are the best described so far and probably also the most serious disorders. Endocrine disruptors that interfere with, for example, thyroid function, glucocorticoid and mineralocorticoid systems, and substances with (anti-)progestagenic effects will also be discussed. Furthermore, substances interacting with PPAR (peroxisome proliferator-activated receptors), RAR and RXR (retinoid receptors), AhR (aryl hydrocarbon / dioxin receptor) or PXR (pregnane X receptor) will be mentioned. Attention will also be paid to natural endocrine disruptors.

Assessment methods and criteria
Oral examination, Seminar work

Oral Exam, Further requirements: semestral project
Recommended literature
  • Escher, Beate Isabella; Leusch, Frederic; Chapman, Heather; Poulsen, Anita. Bioanalytical tools in water quality assessment. First published. London ; New York : IWA-Publishing, 2012. ISBN 978-1-84339-368-9.
  • Norris, D. O., Carr, J. A. Vertebrate Endocrinology (Fifth Edition). 2013. ISBN 9780123948151.
  • Pandian T. J. Environmental Sex Differentiation in Fish. 2015.
  • Pandian, T. J. Reproduction and Development in Crustacea. Boca Raton : CRC Press, Taylor & Francis Group, 2016. ISBN 978-1-4987-4828-5.
  • Pandian, T. J. Reproduction and Development in Mollusca. Boca Raton : CRC Press, Taylor & Francis Group, 2017. ISBN 978-1-1387-1045-0.

Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester