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Lecturer(s)
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Jaklová Kateřina, Mgr.
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Dvorníková Zuzana, Mgr.
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Doudová Lenka, Mgr.
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Tykalová Hana, Mgr. Ph.D.
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Selinger Martin, RNDr. Ph.D.
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Mašková Hana, Mgr.
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Hejduk Libor, Mgr.
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Havlíčková Petra, RNDr.
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Kozelková Tereza, Mgr.
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Šnebergerová Pavla, Mgr.
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Khanna Ritesh
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Tučková Dominika, Ing.
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Kotounová Eliška, Mgr. MSc.
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Course content
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Comparison of protein separation by SDS-PAGE using different concentrations of acrylamide and in gradient gels. Comparison of staining of SDS-PAGE separated proteins using different methods (Ponceau, CBB, silver). Electroblotting of proteins and its use - immunoblotting, lectinoblotting; sensitivity and specificity of immunoblotting; Schiff staining of glycoproteins. Protein labelling using FITC and visualization using SDS-PAGE.
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Learning activities and teaching methods
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Skills training, Laboratory
- Preparation for credit
- 15 hours per semester
- Preparation for classes
- 20 hours per semester
- Class attendance
- 80 hours per semester
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Learning outcomes
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Laboratory course introduces students to selected advanced topics in analytical and preparative biochemistry.
Learning of the advanced methods in biochemistry
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Prerequisites
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Biochemistry Laboratory 1
UCH/758
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Assessment methods and criteria
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Student performance assessment, Test
Active work in the laboratory, preparation for the topics, obtaining at least 50 % points in the tests. Description and explanation of the obtained results during a discussion with a teacher.
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Recommended literature
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