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Vyučující
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Fessl Tomáš, Mgr. Ph.D.
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Sýkora Jan, Mgr. Ph.D.
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Amaro Mariana, Dr. Ph.D.
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Obsah předmětu
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Content of the course: Absorption of light and electronic transitions. Principles of Fluorescence. Principles and Selected Applications of Steady-State and Time-Resolved Fluorescence. Confocal and Two-Photon Fluorescence Microscopy. Recent Developments of Fluorescence Probes for Biosiences. Selected Applications of Fluorescence Microscopy in Cell Biology . Fluorescence in Non-viral Gene Therapy. Photodynamic Diagnosis and Therapy. Diagnosis of Prion Diseases by Fluorescence Correlation Spectroscopy
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Studijní aktivity a metody výuky
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Monologická (výklad, přednáška, instruktáž), Práce s textem (učebnicí, knihou), Pozorování, Individuální příprava ke zkoušce
- Účast na výuce
- 28 hodin za semestr
- Domácí příprava na výuku
- 20 hodin za semestr
- Příprava na zkoušku
- 35 hodin za semestr
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Výstupy z učení
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Principles of fluorescence spectroscopy and microscopy and their applications in biology and medicine Principy fluorescenční spektroskopie a mikroskopie a jejich aplikace v biologii a medicíně.
The course introduces the students to the timely applications of the fluorescence spectroscopy and microscopy in biosciences. The students will be able to design and suggest appropriate fluorescence experiments for the specific biological problem.
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Předpoklady
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The students are to know basics of the optical spectroscopy and microscopy. Moreover, the overview of the structure of biomolecules (DNA, proteins, lipids) and biomolecular self-assemblies (biomembranes, protein associates) is beneficial.
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Hodnoticí metody a kritéria
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Ústní zkouška
The exam is oral. Randomly chosen 2 topics out of 12 is discussed and the understanding of the student to the particular fluorescence approach determines the evaluation.
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Doporučená literatura
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Hof Martin, Hutterer R., Fidler V: Fluorescence Spectroscopy in Biology. pp. 290, Springer Verlag, Heidelberg, 2004 (ISBN 3-540-22338-x).
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Kubitschek U.: Fluorescence Microscopy: From Principles to Biological Applications, Wiley-Blackwell, 1st ed. 2013.
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Lakowitz J.R., Principles of Fluorescence Spectroscopy, Springer, corr. 5th ed., 2010.
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