Course: Advanced Laboratory Course in Biochemistry

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Course title Advanced Laboratory Course in Biochemistry
Course code UCH/050
Organizational form of instruction Lesson
Level of course Bachelor
Year of study not specified
Frequency of the course In each academic year, in the summer semester.
Semester Summer
Number of ECTS credits 4
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Selinger Martin, RNDr. Ph.D.
  • Mašková Hana, Mgr.
  • Loginov Dmitry Sergeevich, Mgr. Ph.D.
  • Kočová Pavlína, Mgr.
  • Hejduk Libor, Mgr.
  • Kotsarenko Kateryna, Mgr. Ph.D.
  • Kolešová Anna, MSc. Ph.D.
  • Štěrba Ján, RNDr. Ph.D.
  • Věchtová Pavlína, RNDr. Ph.D.
  • Havlíčková Petra, Bc.
  • Kozelková Tereza, Mgr.
Course content
Comparison of protein separation by SDS-PAGE using different concentrations of acrylamide and in gradient gels. Comparison of staining of SDS-PAGE separated proteins using different methods (Ponceau, CBB, silver). Electroblotting of proteins and its use - immunoblotting, lectinoblotting; sensitivity and specificity of immunoblotting; Schiff staining of glycoproteins. Protein labelling using FITC and visualization using SDS-PAGE.

Learning activities and teaching methods
Skills training, Laboratory
  • Preparation for classes - 20 hours per semester
  • Preparation for credit - 15 hours per semester
  • Class attendance - 80 hours per semester
Learning outcomes
Laboratory course introduces students to selected advanced topics in analytical and preparative biochemistry.
Learning of advanced methods in biochemistry. Independent work in the laboratory and ability to evaluate the obtained results.
Prerequisites
Biochemistry Laboratory 1
UCH/049
----- or -----
UCH/051

Assessment methods and criteria
Student performance assessment, Test

The course is designed for students in study fields: Chemistry, Chemistry for Teachers
Recommended literature
  • Káš a kol., VŠCHT. Laboratorní techniky biochemie.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Chemistry (1) Category: Chemistry courses 2 Recommended year of study:2, Recommended semester: Summer