| Course title | Advanced Laboratory Course in Biochemistry |
|---|---|
| Course code | UCH/050 |
| Organizational form of instruction | Lesson |
| Level of course | Bachelor |
| Year of study | 2 |
| Frequency of the course | In each academic year, in the summer semester. |
| Semester | Summer |
| Number of ECTS credits | 4 |
| Language of instruction | Czech |
| Status of course | Compulsory |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
|---|
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| Course content |
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Comparison of protein separation by SDS-PAGE using different concentrations of acrylamide and in gradient gels. Comparison of staining of SDS-PAGE separated proteins using different methods (Ponceau, CBB, silver). Electroblotting of proteins and its use - immunoblotting, lectinoblotting; sensitivity and specificity of immunoblotting; Schiff staining of glycoproteins. Protein labelling using FITC and visualization using SDS-PAGE.
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| Learning activities and teaching methods |
Skills training, Laboratory
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| Learning outcomes |
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Laboratory course introduces students to selected advanced topics in analytical and preparative biochemistry.
Learning of advanced methods in biochemistry. Independent work in the laboratory and ability to evaluate the obtained results. |
| Prerequisites |
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Biochemistry Laboratory 1
UCH/049 ----- or ----- UCH/051 |
| Assessment methods and criteria |
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Student performance assessment, Test
Active work in the laboratory, preparation for the topics, obtaining at least 50 % points in the tests. Description and explanation of the obtained results during a discussion with a teacher. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Chemistry (1) | Category: Chemistry courses | 2 | Recommended year of study:2, Recommended semester: Summer |