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Lecturer(s)
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Jaklová Kateřina, Mgr.
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Dvorníková Zuzana, Mgr.
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Doudová Lenka, Mgr.
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Tykalová Hana, Mgr. Ph.D.
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Mašková Hana, Mgr.
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Hejduk Libor, Mgr.
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Havlíčková Petra, RNDr.
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Tučková Dominika, Ing.
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Course content
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Comparison of protein separation by SDS-PAGE using different concentrations of acrylamide and in gradient gels. Comparison of staining of SDS-PAGE separated proteins using different methods (Ponceau, CBB, silver). Electroblotting of proteins and its use - immunoblotting, lectinoblotting; sensitivity and specificity of immunoblotting; Schiff staining of glycoproteins. Protein labelling using FITC and visualization using SDS-PAGE.
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Learning activities and teaching methods
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Skills training, Laboratory
- Preparation for credit
- 15 hours per semester
- Preparation for classes
- 20 hours per semester
- Class attendance
- 80 hours per semester
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Learning outcomes
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Laboratory course introduces students to selected advanced topics in analytical and preparative biochemistry.
Students know and understand the principles of the more advanced biochemical methods (k1, k2), can perform such experiments (k3), analyze the results (k4), and draw conclusions (k5). Students can apply this knowledge and design experiments for needed analyses (k6).
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Prerequisites
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Biochemistry Laboratory 1
UCH/758
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Assessment methods and criteria
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Student performance assessment, Test
Active work in the laboratory, preparation for the topics, obtaining at least 50 % points in the tests. Description and explanation of the obtained results during a discussion with a teacher.
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Recommended literature
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