|Course title||Biochemistry Laboratory 2|
|Organizational form of instruction||Lesson|
|Level of course||Bachelor|
|Year of study||not specified|
|Frequency of the course||In each academic year, in the summer semester.|
|Number of ECTS credits||5|
|Language of instruction||English|
|Status of course||Compulsory|
|Form of instruction||Face-to-face|
|Work placements||This is not an internship|
|Recommended optional programme components||None|
|Course availability||The course is available to visiting students|
Comparison of protein separation by SDS-PAGE using different concentrations of acrylamide and in gradient gels. Comparison of staining of SDS-PAGE separated proteins using different methods (Ponceau, CBB, silver). Electroblotting of proteins and its use - immunoblotting, lectinoblotting; sensitivity and specificity of immunoblotting; Schiff staining of glycoproteins. Protein labelling using FITC and visualization using SDS-PAGE.
|Learning activities and teaching methods|
Skills training, Laboratory
Laboratory course introduces students to selected advanced topics in analytical and preparative biochemistry.
Learning of the advanced methods in biochemistry
Biochemistry Laboratory 1
|Assessment methods and criteria|
Student performance assessment, Test
Active work in the laboratory, preparation for the topics, obtaining sufficient points in the tests. Description and explanation of the obtained results during a discussion with a teacher.
|Study plans that include the course|