Course: Biochemistry Laboratory 2

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Course title Biochemistry Laboratory 2
Course code UCH/642
Organizational form of instruction Lesson
Level of course Bachelor
Year of study not specified
Frequency of the course In each academic year, in the summer semester.
Semester Summer
Number of ECTS credits 5
Language of instruction English
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Course availability The course is available to visiting students
Lecturer(s)
  • Selinger Martin, RNDr. Ph.D.
  • Mašková Hana, Mgr.
  • Loginov Dmitry Sergeevich, Mgr. Ph.D.
  • Kočová Pavlína, Mgr.
  • Hejduk Libor, Mgr.
  • Kotsarenko Kateryna, Mgr. Ph.D.
  • Kolešová Anna, MSc. Ph.D.
  • Štěrba Ján, RNDr. Ph.D.
  • Věchtová Pavlína, RNDr. Ph.D.
  • Havlíčková Petra, Bc.
  • Kozelková Tereza, Mgr.
Course content
Comparison of protein separation by SDS-PAGE using different concentrations of acrylamide and in gradient gels. Comparison of staining of SDS-PAGE separated proteins using different methods (Ponceau, CBB, silver). Electroblotting of proteins and its use - immunoblotting, lectinoblotting; sensitivity and specificity of immunoblotting; Schiff staining of glycoproteins. Protein labelling using FITC and visualization using SDS-PAGE.

Learning activities and teaching methods
Skills training, Laboratory
  • Preparation for credit - 15 hours per semester
  • Preparation for classes - 20 hours per semester
  • Class attendance - 80 hours per semester
Learning outcomes
Laboratory course introduces students to selected advanced topics in analytical and preparative biochemistry.
Learning of the advanced methods in biochemistry
Prerequisites
Biochemistry Laboratory 1
UCH/758

Assessment methods and criteria
Student performance assessment, Test

Active work in the laboratory, preparation for the topics, obtaining sufficient points in the tests. Description and explanation of the obtained results during a discussion with a teacher.
Recommended literature


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Biological Chemistry (1) Category: Chemistry courses 2 Recommended year of study:2, Recommended semester: Summer