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Lecturer(s)
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Durchan Milan, RNDr. CSc.
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Course content
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Content of lectures: Fundamentals of electromagnetic theory of light. Blackbody radiation. Photons, the photoelectric effect. Elementary quantum process of light emission. Excited state lifetime, intensity and width of a spectral lines. Light sources and detectors. Dispersive optical elements. Fundamentals of rotational, vibrational and electronic spectroscopy. Methods absorption spectroscopy. Optical activity, linear and circular dichroism. Raman scattering and infrared spectroscopy. Time resolved spectroscopy. Emission methods. Thermoluminescence. Luminescence in biology. Theory of excitation energy transfer. Methods of laser spectroscopy. Content of practicals: Dispersion and spectral properties of the monochromator. FWHM of bands, Gauss and Lorentz profile, the second derivative. Absorption of transparent samples. Absorption of light-scattering samples. Measurement of fluorescence emission and excitation spectra, mixture of photosynthetic pigments as sample. Measuring the relative fluorescence quantum yield. Fluorescence polarization. Transfer of excitation energy. Circular dichroism.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Demonstration, Laboratory
- Class attendance
- 28 hours per semester
- Preparation for exam
- 28 hours per semester
- Preparation for classes
- 10 hours per semester
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Learning outcomes
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This course introduces methods of optical spectroscopy used primarily for the study of organic molecules and biological systems. Lecturer will demonstrate the theoretical fundamentals and the methods were applied at limit frontier of theoretical availability. Instrumentation is involved in practicals.
Students intensify their knowledge of optics, optical instrumentation techniques and methods used primarily for biological materials.
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Prerequisites
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Basic knowledge of Physics III
UFY/FYZ3K and UFY/FYZ3
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Assessment methods and criteria
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Oral examination, Development of laboratory protocols
Part of the course Optical Spectroscopy is a regular, one-week, practice. All protocols on practices are required. Oral exam.
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Recommended literature
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Amesz J., Hoff A.J.(eds.): Biophysical Techniques in Photosynthesis. Kluwer, 1996.
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Beiser A.: Úvod do moderní fyziky.Academia,Praha 1975.
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Brož J. a kol.: Základy fyzikálních měření, SPN Praha 1967.
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.Prosser V.a kol.: Experimentální metody biofyziky. Academia Praha, 1989.
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Saleh B.E.A., Teich M.C.: Základy fotoniky (svazek 1-4), Matfyzpress, Praha 1994.
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