Biochemical laboratory 5 weeks of practice 1. Internal and external inspection of quality of examination in a biochemical laboratory 2. Preparatory and auxiliary work: disinfection, sterilization, cleaning of laboratory equipment 3. Examination of urine: extraction systems, quantity, colour, smell, nebula, foam, biochemical composition (proteins, glycides, keto-compounds, bile pigments, bilirubin, urobilinogen, urobilin haemoglobin, phenyl-acetylformic acid, false cast (blood corpuscles, epithelia, cylinders, crystals) 4. Inspection of pre-analytical stage 5. Examination of stool 6. Determination of serum proteins 7. Urine, creatinine and creatine, urinary acid in serum 8. Amino-acids in serum 9. Lipids in serum 10. Saccharides in serum 11. OGTT 12. Enzymes 13. Inorganic substances in serum 14. Selected immunochemical examinations 15. Examination of acid-base equilibrium 16. Examination of cerebrospinal fluid 17. Toxicological examinations Immunohaematology and transfusion service 2 weeks of practice 1. Organisation and management of laboratory operation. SMJ, SVP a work with LIS. 2. Laboratory examination of donors of blood 3. Laboratory test for blood groups and blood compatibility 4. Examination of antibodies 5. Examination of leucocytic and thrombocytic antigens 6. Immunohaematological examinations concerning HDN (haemolytic disease of the new-born) 7. Examination for elimination of infectious diseases transmission hazard due to transfusion equipment Genetic laboratory (clinic genetics) 1 week - Cytogenetics cultivation methods and direct methods, banding, G, C, NOR; Hybridization techniques FISH, mFISH, CGH. Examination of per. blood, bone marrow, tumour tissue. Assessment of mosaics, clones. - Molecular genetics extra-human genome: isolation of DNA/RNA from various sources, detection of viral NA, detection of bacterial 16S, identification of sequences by sequential analysis, quantitative analysis of viral loads (Real Time PCR), interpretation (comparisons and links to other microbiological methods). - Molecular genetics human genome: isolation of DNA/RNA, PCR detection SNP (end point and Real Time PCR), sequencing, detection of hypermutations in lgH in CLL, quantification expression (bcr/abl), interpretation and the issue of intergeneration validity of molecular genetic examinations. Protection of public health 1 week - Drinking water sampling: determination of free chlorine, pH, conductivity, organoleptic properties (smell, taste) and elaborating the protocol - Surface water sampling from a stream or a pond: determining the temperature, pH, conductivity, transparency and elaborating the protocol - Measuring the quality of indoor environments, CO2, temperature, relative humidity in school facilities for the period of teaching process 6-7 hours compared to the air quality in outdoor environments, elaborating the protocol - Measuring the quality of indoor catering working environments: such as the kitchen. The effect of the number of employees on temperature and humidity, the size of the kitchen, shift length, elaborating the protocol and attaching photographic documentation.
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