Course: Practical in Clinical Laboratory 4

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Course title Practical in Clinical Laboratory 4
Course code ULZ/HHPR4
Organizational form of instruction Seminary
Level of course Bachelor
Year of study 3
Semester Summer
Number of ECTS credits 9
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Kašparová Marie, Ing.
Course content
Biochemical laboratory 5 weeks of practice 1. Internal and external inspection of quality of examination in a biochemical laboratory 2. Preparatory and auxiliary work: disinfection, sterilization, cleaning of laboratory equipment 3. Examination of urine: extraction systems, quantity, colour, smell, nebula, foam, biochemical composition (proteins, glycides, keto-compounds, bile pigments, bilirubin, urobilinogen, urobilin haemoglobin, phenyl-acetylformic acid, false cast (blood corpuscles, epithelia, cylinders, crystals) 4. Inspection of pre-analytical stage 5. Examination of stool 6. Determination of serum proteins 7. Urine, creatinine and creatine, urinary acid in serum 8. Amino-acids in serum 9. Lipids in serum 10. Saccharides in serum 11. OGTT 12. Enzymes 13. Inorganic substances in serum 14. Selected immunochemical examinations 15. Examination of acid-base equilibrium 16. Examination of cerebrospinal fluid 17. Toxicological examinations Immunohaematology and transfusion service 2 weeks of practice 1. Organisation and management of laboratory operation. SMJ, SVP a work with LIS. 2. Laboratory examination of donors of blood 3. Laboratory test for blood groups and blood compatibility 4. Examination of antibodies 5. Examination of leucocytic and thrombocytic antigens 6. Immunohaematological examinations concerning HDN (haemolytic disease of the new-born) 7. Examination for elimination of infectious diseases transmission hazard due to transfusion equipment Genetic laboratory (clinic genetics) 1 week - Cytogenetics cultivation methods and direct methods, banding, G, C, NOR; Hybridization techniques FISH, mFISH, CGH. Examination of per. blood, bone marrow, tumour tissue. Assessment of mosaics, clones. - Molecular genetics extra-human genome: isolation of DNA/RNA from various sources, detection of viral NA, detection of bacterial 16S, identification of sequences by sequential analysis, quantitative analysis of viral loads (Real Time PCR), interpretation (comparisons and links to other microbiological methods). - Molecular genetics human genome: isolation of DNA/RNA, PCR detection SNP (end point and Real Time PCR), sequencing, detection of hypermutations in lgH in CLL, quantification expression (bcr/abl), interpretation and the issue of intergeneration validity of molecular genetic examinations. Protection of public health 1 week - Drinking water sampling: determination of free chlorine, pH, conductivity, organoleptic properties (smell, taste) and elaborating the protocol - Surface water sampling from a stream or a pond: determining the temperature, pH, conductivity, transparency and elaborating the protocol - Measuring the quality of indoor environments, CO2, temperature, relative humidity in school facilities for the period of teaching process 6-7 hours compared to the air quality in outdoor environments, elaborating the protocol - Measuring the quality of indoor catering working environments: such as the kitchen. The effect of the number of employees on temperature and humidity, the size of the kitchen, shift length, elaborating the protocol and attaching photographic documentation.

Learning activities and teaching methods
Learning outcomes
Student find basic biochemical laboratory method and methods from region immunohaematology and transfusive services in laboratory.
Student find basic biochemical laboratory method and methods from region immunohaematology and transfusive services in laboratory.

Assessment methods and criteria
Student performance assessment

The student's active participation in the practice.
Recommended literature
  • Milan Dastych a kol. Klinická Biochemie. Masarykova univerzita, Brno, 2008.

Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Health and Social Sciences Study plan (Version): - (2) Category: Health service 3 Recommended year of study:3, Recommended semester: Summer