| Course title | Methods of microbial diagnostics |
|---|---|
| Course code | KME/065 |
| Organizational form of instruction | Lecture + Lesson |
| Level of course | Master |
| Year of study | not specified |
| Frequency of the course | In each academic year, in the winter semester. |
| Semester | Winter |
| Number of ECTS credits | 5 |
| Language of instruction | Czech |
| Status of course | Compulsory-optional |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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Content of lectures: 1. Microscopic methods, staining techniques in microbiology, 2. Virological and bacteriological cultivation methods, classical typing methods, 3. Biochemical methods of species identification of bacteria, determination of sensitivity to antibiotics, 4. Molecular biological methods for identification of microorganisms (hybridization techniques, restriction analysis, microbial genome mapping and construction of gene libraries, sequencing methods, polymerase chain reaction, electrophoresis of nucleic acids, genotyping), 5. Proteomic methods for microbial diagnostics (protein electrophoresis, blotting techniques and other electromigration methods, mass spectrometry of peptides and proteins, flow cytometry), 6. Immunological methods for microorganism identification (using polyclonal, monoclonal and recombinant antibodies, immunomagentic seapartion, agglutination tests, immunoassays (ELISA, RIA, FIA) biosensor technology, dip-stick techniques and other methods for rapid diagnosis of microorganisms).
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| Learning activities and teaching methods |
Monologic (reading, lecture, briefing), Demonstration
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| Learning outcomes |
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The main objective of the course is to provide a comprehensive overview of microbiological, biochemical, molecular biological and immunological methods for the diagnostics of pathologically significant microorganisms in human medicine.
Students understand the principles of microbiological, biochemical, molecular-biological and immunological methods for diagnostics of medically important pathogens. |
| Prerequisites |
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Se the list of prerequisites. Prerequisites are compulsory.
KBE/260 and KMB/250 and UCH/CV036 ----- or ----- UCH/036 |
| Assessment methods and criteria |
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Oral examination
Student must have knowledge of known methods of microbial diagnostics and should be able to suggest suitable method. The exam is oral, 2 out of 4 questions must be answered |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Clinical Biology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Winter |