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Lecturer(s)
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Vácha František, prof. RNDr. Ph.D.
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Kutá Smatanová Ivana, prof. Mgr. Ph.D.
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Kutý Michal, Mgr. Ph.D.
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Kaščáková Barbora, Mgr. Ph.D.
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Havlíčková Petra, RNDr.
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Course content
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Content of Lectures: Chemical structure and geometry, conformation and energy (Michal Kutý) Structure of proteins (Ivana Kutá Smatanová, Michal Kutý) Structure of nucleic acids (Tomáš Fessl) Structure of polysaccharides and biological membranes (František Vácha) Computational methods and molecular modelling (Zdeněk Futera) X-ray crystallography (Pavlína Řezáčová) Nuclear Magnetic Resonance (Vašek Veverka) Electron microscopy (Zdenko Gardian) Mass spectroscopy (Filip Dyčka, Peter Koník) Absorption and fluorescence spectroscopy (Radek Litvín) Optical microscopy and CD (Tomáš Fessl) New trends in structural biochemistry (Ivana Kutá Smatanová) Content of Practicals: (Barbora Kaščáková, Petra Havlíčková) The knowledge acquired during the lectures will be deepened during the exercise. Solving the structure of a selected protein, visualization using Pymol, using a program for structure prediction (AlphaFold)
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Work with text (with textbook, with book), Individual tutoring
- Preparation for credit
- 14 hours per semester
- Preparation for exam
- 14 hours per semester
- Class attendance
- 56 hours per semester
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Learning outcomes
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The lectures will give students an insight into why it is important to understand the function of macromolecular structures at the molecular level. There have been breakthroughs in modern biochemistry with the development of techniques such as XRD, NMR and EM that allow the structures of macromolecules to be determined. Today, students commonly use structural models of proteins and nucleic acids available in computer databases. These models provide an illustrative but often idealized representation of biological macromolecules. It is therefore crucial that students of modern biochemistry become familiar with structural analysis methods during their studies. By doing so, they will be able to distinguish between what has been measured and what has been calculated and thus better understand the difference between reality and the model.
Students will acquire theoretical knowledge of functional and structural biochemistry as well as practical skills involving the use of various methodological procedures and the use of many modern instrumental methods in the study of the structure and function of living organisms at the molecular level.
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Prerequisites
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The minimum requirement for students is basic knowledge of chemistry and biochemistry acquired after completing university education of biological, chemical or biochemical specialization.
UCH/100 and UCH/101 and KCH/OCH1C and KCH/OCH2C and UCH/032
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Assessment methods and criteria
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Written examination, Combined exam
Credit will be awarded on the basis of a short lecture on a professional article that students will be given to study. The final exam will take the form of oral interview with the final evaluation in the form of awarding the mark.
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Recommended literature
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Kensal E. van Holde, W. Curtis Johnson, P. Shing Ho: Principles of physical biochemistry 2nd ed. Pearson Education, Inc. (2006), ISBN 0-13-046427-9.
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Žídek, L. a kol.: Strukturní biochemie, MU Brno, 2015.
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