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Vyučující
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Sedláček Zdeněk, prof. Ing. DrSc.
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Riegert Bystřická Dagmar, Mgr. Ph.D.
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Vaněček Tomáš, RNDr. Ph.D.
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Obsah předmětu
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nespecifikováno
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Studijní aktivity a metody výuky
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nespecifikováno
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Výstupy z učení
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In this practical course, students will implement basic DNA diagnostic methods (PCR, qPCR, Sanger sequencing) in clinical genetic setting and learn advanced cytogenetic methods (Fluorescent in-situ hybridization (FISH)) and next generation sequencing (NGS) approaches to diagnosis
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Předpoklady
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nespecifikováno
KMB/932 a zároveň KMB/608
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Hodnoticí metody a kritéria
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nespecifikováno
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Doporučená literatura
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Theda, C., Hwang, S.H., Czajko, A. et al. Quantitation of the cellular content of saliva and buccal swab samples. Sci Rep 8, 6944 (2018). https://doi.org/10.1038/s41598-018-25311-0.
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Biolanalytics: Analytical Methods anad Concepts in Biochemistry and Molecular Biology. Friedrich Lottspeich, Joachim W. Engels, Wiley-VCH Verlag GmbH 2018.
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Chromosomal Abnormalities - A Hallmark Manifestation of Genomic Instability. InTech. DOI: 10.5772/67415. Due to the fast evolving character of the next generation sequencing approaches, students will be provided with upto-date literature in form of instructive reveiws regularly published in Lancet, JAMA and NEJM journals at the beginning of the course..
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Kalkan R (2017) The Use of Molecular Cytogenetic Techniques for the Identification of Chromosomal Abnormalities.
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