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Vyučující
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Nguyen Petr, RNDr. Ph.D.
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Gahurová Lenka, Mgr. Ph.D.
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Moos Martin, Ing. Ph.D.
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Chodáková Lenka, Mgr. Ph.D.
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Obsah předmětu
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Content of tutorials/seminar: Genomics - library preparation, genome sequencing using nanopore technology, data analysis (3 days) Transcriptomics - RNA isolation, library preparation and sequencing using Illumina, data analysis (3 days) Proteomics - proteomics facility tour and proteomics data analysis (1 day) Metabolomics - metabolomics facility tour and metabolomics data analysis (1 day) CRISPR-Cas9 yeast genome editing - gRNA design, plasmid cloning and yeast transformation, phenotype analysis (4 days)
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Studijní aktivity a metody výuky
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- Účast na výuce
- 50 hodin za semestr
- Příprava na zápočet
- 25 hodin za semestr
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Výstupy z učení
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These are 5 block practicals in the last 3 weeks of the semester covering the most commonly used OMICs approaches and gene editing. Three of the multi-day practicals are truly hands-on, two are guided visits to service facilities with the opportunity to try out data analysis.
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Předpoklady
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nespecifikováno
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Hodnoticí metody a kritéria
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nespecifikováno
Credit: attendance and approved protocols from the practicals
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Doporučená literatura
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Detailed protocols for practicals.
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