| Název předmětu | Cytogenomics |
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| Kód předmětu | KMB/935 |
| Organizační forma výuky | Přednáška + Cvičení |
| Úroveň předmětu | Magisterský |
| Rok studia | nespecifikován |
| Četnost výuky | V každém akademickém roce, jen v zimním semestru. |
| Semestr | Zimní |
| Počet ECTS kreditů | 4 |
| Vyučovací jazyk | angličtina |
| Statut předmětu | nespecifikováno |
| Způsob výuky | Kontaktní |
| Studijní praxe | Nejedná se o pracovní stáž |
| Doporučené volitelné součásti programu | Není |
| Vyučující |
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| Obsah předmětu |
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Content of lectures: 1-2 Chromosome Structure and Function Mitosis, meiosis, anatomy of chromosomes, including structural components, centromeres, telomeres, genome composition and repetitive sequences, banding techniques. 3 Fluorescent In Situ Hybridization (FISH) FISH principles, applications in cytogenetics, advanced FISH techniques, fluorescence microsopy 4 Array Comparative Genomic Hybridization (aCGH) Principles of aCGH, clinical applications in cytogenomics 5-6 Next-Generation Sequencing (NGS) in Cytogenomics Recapitulation of NGS technologies, their impact on understanding genomic variation, epigenomic landscapes, genome composition, Oligopaint 7 3D Nuclear Organization Basics of 3D nuclear organization, chromatin structure, methodologies (e.g., Hi-C) 8 Chromatin and Epigenomics Definitions and basic concepts of epigenetics, including DNA methylation, histone modifications, non-coding RNAs 9-10 Epigenomic Landscapes Exploration of large-scale epigenomic studies, covering techniques such as ChIP-seq (Chromatin Immunoprecipitation sequencing), DNA methylation profiling, ATAC-seq 11-12 Repetitive Sequences Understanding repetitive sequences in the genome, including transposons, satellite DNA, and tandem repeats. Exploration of methodologies for repetitive sequence analysis. 13 Applications of Cytogenomics Cytogenomics in genetic disorders and cancer, personalized medicine Content of tutorials: one week's block of practicals will supplement the lectures and it will include modern molecular cytogenetic methods such as fluorescence in situ hybridization with oligo paint probes and immunodetection of heterochromatin markers.
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| Studijní aktivity a metody výuky |
| nespecifikováno |
| Výstupy z učení |
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The Cytogenomics course aims to provide students with a foundational understanding of modern cytogenetic and cytogenomic approaches. It explores advanced genomic technologies and their applications chromosome biology studies of, 3D nuclear organization, epigenetics, and genome composition. Students will learn how are cytogenomic techniques applied in diagnosing genetic disorders and cancer.
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| Předpoklady |
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nespecifikováno
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| Hodnoticí metody a kritéria |
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nespecifikováno
Credit: attendance at the practicals, presentation of selected applications and case studies in cytogenomics Exam: oral and written exam (min. 50%) |
| Doporučená literatura |
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| Studijní plány, ve kterých se předmět nachází |
| Fakulta | Studijní plán (Verze) | Kategorie studijního oboru/specializace | Doporučený semestr |
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