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Vyučující
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Čurn Vladislav, prof. Ing. Ph.D.
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Obsah předmětu
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Content of lectures: The main topics of the course by individual weeks (13 weeks per semester) of teaching. History, achievements and perspectives of plant breeding, phenotypic and genotypic selection, Climate Smart Plant Breeding (1) Plant breeding process, genetic structure of varieties (2) Plant genetic resources, origin and domestication of crops, conservation of genetic diversity (3) Methods of plant breeding, intraspecific and interspecific crossing, crossing technique (4-5) Hybrid, mutant and polyploid breeding (6) Biotechnological methods in plant breeding (7-8) Case study - Application of biotechnological methods in potato breeding (9) Case study - Application of biotechnological methods in rape breeding (10) Molecular breeding, marker assisted selection, associative transcriptomics in plant breeding (11-12) Transgenes useful for crop improving, NBT - New Breeding Technology, cisgenesis, gene editing (13) Content of tutorials: Laboratory practicals: Analysis of morphological traits, phenotyping, yield factor analysis, selection parameters Potato breeding for quality, starch quality evaluation, table value evaluation Bread wheat breeding and bakery quality parameters evaluation, gluten quantity and quality analysis, detection of presence of Rht genes Rape breeding, fatty acid composition, glucosinolate analysis from single cotyledon Molecular approaches in plant breeding - analysis of microspore derived embryos, identification of variety purity, genomics and transcriptomics in plant breeding, detection of genes of interest and genotypic selection
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Studijní aktivity a metody výuky
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- Příprava na zápočet
- 16 hodin za semestr
- Účast na výuce
- 59 hodin za semestr
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Výstupy z učení
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The aim of the course is to provide knowledge about biotechnological applications in genetic resources management aand crop improvement, as well as the use of modern molecular biological methods in plant breeding.
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Předpoklady
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nespecifikováno
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Hodnoticí metody a kritéria
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nespecifikováno
Credit: attendance at the laboratory practicals (max. 3 absences), completing laboratory protocols. Written exam (min. 50 %)
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Doporučená literatura
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Alberts B.: Molecular Biology of the Cell. W. W. Norton & Company, 2017..
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Al-Khayri J. M., Jain S. M., Johnson D. V.: Advances in Plant Breeding Strategies: Breeding, Biotechnology and Molecular Tools. Springer, 2015..
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Bradshaw J. E.: Plant Breeding: Past, Present and Future. Springer, 2016..
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Fleury D., Whitford R.: Crop Breeding. Springer, 2014..
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Lesk A.: Introduction to Genomics. Oxford Univ. Press. 2017..
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Reece,R.J.: Analysis of Genes and Genomes. Wiley Publishing, 2004..
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