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Vyučující
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Selinger Martin, RNDr. Ph.D.
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Vácha František, prof. RNDr. Ph.D.
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Franta Zdeněk, RNDr. Ph.D.
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Dyčka Filip, Mgr. Ph.D.
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Fessl Tomáš, Mgr. Ph.D.
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Obsah předmětu
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1. Introduction, terminology, basic principles of molecular cloning 2. Recombinant DNA enzymes 3. Introduction of DNA into host cells 4. Prokaryotic expression systems 5. Eukaryotic expression systems 6. Strategies of protein engineering 7. In vitro mutagenesis 8. Recombinant proteins 9 . Protein-protein interactions 10. Methods of gene targeting 11. Transgenic organisms 12. Molecular medicine
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Studijní aktivity a metody výuky
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Monologická (výklad, přednáška, instruktáž), Demonstrace, Laboratorní práce
- Domácí příprava na výuku
- 100 hodin za semestr
- Účast na výuce
- 39 hodin za semestr
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Výstupy z učení
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Genetic engineering, the specific and directed alteration of an organism´s hereditary material, have changed modern biology and biotechnology. There are thousands applications of using of bacteria, yeasts or insect cells of the production of recombinant proteins and various chemical compounds, many of them on the industrial scale. Protein engineering, a specific part of genetic engineering, is the process of developing useful or valuable proteins. It is a young discipline, with much research currently taking place into the understanding of protein folding and protein recognition for protein design principles. Both rational protein design and directed evolution techniques based on random mutagenesis are employed to generate molecules with novel properties. The course focuses on the molecular and genetic tools used to analyze and modify genetic material and to modify organisms to produce desired molecules and proteins.
Students master theoretical knowledge about construction of genetically modified bacteria, plants and animal and about development of new proteins using protein engineering. Students gain insight into applications of genetic and protein engineering. In practical part, students acquire skills in cloning a gene into expression vector, codon optimization, fusion construct generation, protein mutations and evaluation of new protein properties.
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Předpoklady
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Basic knowledge of biochemistry, genetics and molecular and cell biology. The knowledge of basic bioinformatics, working with the sequences, 3D models of proteins. The course builds on the knowledge gained in UCH013/UCH013E and UCH/011.
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Hodnoticí metody a kritéria
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Analýza výkonů studenta
Understanding the principles of all aspects covered by lectures. The course includes practical session in form of homeworks. Individual homeworks will be assigned by the teacher at the end of the lecture. Attendance at the introductory lecture is mandatory. Students are required to complete all assigned homeworks and submit them prior deadline (announced by respective teacher at the end of individual lecture). The teacher will evalute the submited homeworks and only students who successfully complete min 75% of homeworks are allowed to take the final test. There will be two tests during the semestr. One mid-term test with an unknown date, and the final test one week after the last lecture. The mid-term test is not mandatory and will provide bonus points (Bonus points from the mid-term test will improve your mark from the final test up to 10 %). The final test is mandatory for everyone and will be marked according to rules introduced to the students during the first lecture Writting exam, 50% points as a minimum
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Doporučená literatura
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Glick, R., Pasternak, J.J., Patten, C.L. Molecular Biotechnology. 2010. ISBN 978-1-55581-498-4.
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