Course: Plant Biotechnology

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Course title Plant Biotechnology
Course code KGZB/RB
Organizational form of instruction Lecture + Lesson
Level of course Master
Year of study 1
Semester Winter and summer
Number of ECTS credits 5
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Čurn Vladislav, prof. Ing. Ph.D.
  • Beran Pavel, Ing. Ph.D.
  • Jozová Eva, Ing. Ph.D.
Course content
Historical overview of the methodology plant tissue cultures. Aseptic cultures of plants, embryos, organs, tissues, cells and protoplasts. Principles of aseptic handling of plant material. Nutrition of in vitro cultures. Hormonal requirements of cultures. Physical cultivation conditions. Regulation of morphogenesis. Basic processes of regeneration in plant in vitro cultures. Genetic and phenotypic variability in in vitro cultures. Somaclonal variability. Production and use of haploid cultures and regenerants. Protoplast culture, somatic hybridization. Cryopreservation. Possibilities and prospects of application of biotechnology in crop improvement (cereals, pulses, oilseeds, potatoes, seed and root crops, legumes and forage grasses, vegetables, hops, ornamental plants, forest and fruit trees). Princeiples of DNA recombinant technology and plant genetic engineering. Transgenosis of plants via Agrobacterium. Integration of T-DNA into plant genome. T-DNA genes, the mechanism of T-DNA integration. Direct introduction of genes into plant genome. Transformation of protoplasts, tissue and organ culture, electroporation, microinjection, mikroprojectiles. Transgenes important for plant breeding. Ethical and legal issues of genetic engineering.

Learning activities and teaching methods
Monologic (reading, lecture, briefing)
  • Preparation for credit - 10 hours per semester
  • Preparation for exam - 20 hours per semester
  • Semestral paper - 20 hours per semester
  • Class attendance - 56 hours per semester
Learning outcomes
The aim of the subject is to acquaint students with current research trends in plant biotechnology, modern biotechnology processes.
Students are able to orientate in the field of plant biotechnology
Standard knowledge of biology

Assessment methods and criteria
Oral examination

Active participation in exercises, protocols from laboratory exercises, understanding the curriculum defined for the exam.
Recommended literature
  • Čurn, V. Rostlinné biotechnologie, I. Fyziologie a genetika rostlinných explantátových kultur..
  • Čurn, V. Rostlinné biotechnologie, II. APlikace biotechnologických metod ve lšechtitelských postupech..
  • Kováč,J. Explantátové kultury rostlin, skripta. Univeverzita J.E.Purkyně, 1992.
  • Novák, F.J. Explantátové kultury. Praha, Academica 1990, 208s..
  • Ondřej, M., Drobník, J. Tranzgenoze rostlin, Academia Praha, 2002, 316s..
  • Ondřej, M. Genové inženýrství kulturních rostlin. Praha, Academia 1992, 232s..
  • Primrose, S.B. Molecular Biotechnology. Oxford, Blackwell Sci. Publ., 1991, 196s..
  • Son, W.-Y., Bhojwani, S.S. Morphogenesis in plant tissue cultures. Kluwer Academic Publishers..
  • Trigiano, R.N., Gray, D.J. Plant development and biotechnology. CRC Press Boca Raton, 358s..

Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Agriculture Study plan (Version): Agricultural Biotechnology (2017) Category: Agriculture and forestry 1 Recommended year of study:1, Recommended semester: Winter
Faculty: Faculty of Agriculture Study plan (Version): Biology and protection of "hobby" organisms (2019) Category: Agriculture and forestry 1 Recommended year of study:1, Recommended semester: Winter
Faculty: Faculty of Agriculture Study plan (Version): Agricultural Products´ Quality (2017) Category: Agriculture and forestry 2 Recommended year of study:2, Recommended semester: Winter