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Lecturer(s)
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Fencková Michaela, Mgr. Ph.D.
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Janoštiak Radislav, RNDr. Ph.D.
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Ellederová Zdeňka, Ing. Ph.D.
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Course content
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Content of lectures: Introduction into research of disease mechanisms Protein structure: crystallography and structural modelling of disease variants Protein-protein interactions: mass spectroscopy, cryoEM, cell-based assays Genome instability and malignant transformation: next generation sequencing and functional cell-based assays to identify and study new cancer genes Mechanisms that drive cell proliferation and quiescence in cancer: 3D organoids, patient cell-lines, CRISPR-Cas9 Cellular and zebrafish models of ciliopathies Function and pharmacology of ionotropic receptors: cellular neurophysiology, biophysics and zebrafish models of epilepsy and neurodevelopmental disorders Investigation of mitochondrial disorders with patient-derived cell lines, rat and mouse models C.elegans as a model for development and cell signalling in health and disease Drosophila as a model of human disease with specific focus on neurodevelopmental disorders Czech Centre for Phenogenomics and genetically modified mouse models of disease: genetic engineering, advanced phenotyping and imaging Non-traditional models: minipig model for Huntington disease Precision medicine to maximize the effectiveness of treatment and prevention Content of tutorials/seminar: For each seminar, students will prepare a presentation of a scientific article that covers one of the lectures´ topics and that will be discussed in the class.
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Learning activities and teaching methods
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unspecified
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Learning outcomes
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This course acquaints students with state-of-the-art experimental genetic and computational approaches in translational research of human disease. The approaches will be presented by experts in the field who will include examples of their current research. Each lecture will be coupled with a seminar where the students will process up-todate literature and discuss it with the rest of the class. Finally, the theoretical knowledge will be complemented with an excursion to the laboratories of the leading research institutes of the Czech Academy of Sciences (IMG, BIOCEV, IPHYS). This concept will broaden the students' perspectives for choosing research interest for possible further exploration during their Master studies, and reinforce their critical thinking and problem-solving.
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Prerequisites
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Genetics, Molecular Biology (KBM/250 or equivalent course from former university).
KMB/250 and KMB/240
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Assessment methods and criteria
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unspecified
Requirements on student: credit: attendance at practicals and seminars (max. 3 absences), presentation 1x semester exam: written (50 %)
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Recommended literature
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Lecturers will provide students with scientific articles that the students will present at the seminars.
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William B. Coleman and Gregory J. Tsongalis: Molecular pathology, 2nd edition, 2018.
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