Course: Methods of microbial diagnostics

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Course title Methods of microbial diagnostics
Course code KME/065
Organizational form of instruction Lecture + Lesson
Level of course Master
Year of study not specified
Frequency of the course In each academic year, in the winter semester.
Semester Winter
Number of ECTS credits 5
Language of instruction Czech
Status of course Compulsory-optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Eyer Luděk, RNDr. Ph.D.
Course content
Content of lectures: 1. Microscopic methods, staining techniques in microbiology, 2. Virological and bacteriological cultivation methods, classical typing methods, 3. Biochemical methods of species identification of bacteria, determination of sensitivity to antibiotics, 4. Molecular biological methods for identification of microorganisms (hybridization techniques, restriction analysis, microbial genome mapping and construction of gene libraries, sequencing methods, polymerase chain reaction, electrophoresis of nucleic acids, genotyping), 5. Proteomic methods for microbial diagnostics (protein electrophoresis, blotting techniques and other electromigration methods, mass spectrometry of peptides and proteins, flow cytometry), 6. Immunological methods for microorganism identification (using polyclonal, monoclonal and recombinant antibodies, immunomagentic seapartion, agglutination tests, immunoassays (ELISA, RIA, FIA) biosensor technology, dip-stick techniques and other methods for rapid diagnosis of microorganisms).

Learning activities and teaching methods
Monologic (reading, lecture, briefing), Demonstration
  • Preparation for classes - 30 hours per semester
  • Class attendance - 66 hours per semester
  • Preparation for exam - 30 hours per semester
Learning outcomes
The main objective of the course is to provide a comprehensive overview of microbiological, biochemical, molecular biological and immunological methods for the diagnostics of pathologically significant microorganisms in human medicine.
Students understand the principles of microbiological, biochemical, molecular-biological and immunological methods for diagnostics of medically important pathogens.
Prerequisites
Se the list of prerequisites. Prerequisites are compulsory.
KBE/260 and KMB/250 and UCH/036

Assessment methods and criteria
Oral examination

Student must have knowledge of known methods of microbial diagnostics and should be able to suggest suitable method. Exam is oral and evaluation individual.
Recommended literature
  • JURÁNKOVÁ, Jana. Klinická mikrobiologie v laboratorní praxi. Bakalářský obor Zdravotní laborant. 1. vyd. Brno: Masarykova univerzita, 2011. 73 s. ISBN 978-80-210-5657-2..
  • KNOZ, Jan a Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992. 195 s. ISBN 80-210-0473-8.
  • PRICE, Christopher, P., a NEWMAN, David, J. (Ed.). Principles and Practices of Immunoassay. 2. vyd. London: Macmillan Reference LTD, 1997, 667 s., ISBN 1-56159-145..
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ a Jana KOPTÍKOVÁ. Metody molekulární biologie. 1. vyd. Brno: Masarykova univerzita, 2005. 194 s. 1. vydání. ISBN 80-210-3841-1..
  • VEENSTRA, Timothy, D. a YATES John, R. (Ed.). Proteomics for Biological Discovery. New Jersey: John Wiley and Sons, Inc, 2006, 326 s, ISBN 978-0-471-16005-2..
  • VOTAVA, Miroslav, Filip RŮŽIČKA, Vladana WOZNICOVÁ, Lenka ČERNOHORSKÁ, Milada DVOŘÁČKOVÁ, Monika DVOŘÁKOVÁ HEROLDOVÁ, Veronika HOLÁ a Ondřej ZAHRADNÍČEK. Lékařská mikrobiologie: Vyšetřovací metody. 1. vyd. Brno: Neptun, 2010. 495 s. ISBN 978-80-86850-04-7..
  • WILD, David (Ed.). The Immunoassay Handbook. 2. vyd. New York: Nature Publishing Group, 2001, 906 s., ISBN 1-56159-270-6..
  • J. B. Carter et al. Virology: Principles and Applications. 2013.
  • M. Votava et al. Lékařská mikrobiologie: Vyšetřovací metody. 1st edition. Neptun, 2010.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Clinical Biology (1) Category: Biology courses - Recommended year of study:-, Recommended semester: Winter