Course: Structural Biochemistry

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Course title Structural Biochemistry
Course code UCH/014E
Organizational form of instruction Lecture + Lesson
Level of course Doctoral
Year of study not specified
Frequency of the course In each academic year, in the summer semester.
Semester Summer
Number of ECTS credits 4
Language of instruction English
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Kutá Smatanová Ivana, prof. Mgr. Ph.D.
  • Kutý Michal, Mgr. Ph.D.
Course content
Content of lectures: 1. Chemical structure and geometry 2. Conformation and energy 3. Structure of proteins and nucleic acids 4. Structure of polysaccharides and biological membranes 5. Calculation methods and molecular modeling 6. Preparation of samples of biomacromolecules, sequencing, isolation, purification, synthesis 7. X-ray crystallography 8. Nuclear magnetic resonance 9. Electron microscopy 10. Mass spectroscopy 11. Optical microscopy and CD 12. Absorption and fluorescence spectroscopy 13. Bioinformatics Content of practicals: During the course the knowledge gained during the lectures will be enhanced.

Learning activities and teaching methods
Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Work with text (with textbook, with book), Individual tutoring
  • Preparation for credit - 14 hours per semester
  • Preparation for exam - 14 hours per semester
  • Class attendance - 56 hours per semester
Learning outcomes
The lectures will explain why the knowledge of molecular structure is important for the precise understanding of the of macromolecular structures function. A breakthrough in modern biochemistry has occurred with the development of techniques to determine the structure of macromolecules (XRD, NMR, EM), and it is currently common place that students use structural models of proteins and nucleic acids available in computer databases for their work where the representation of biological macromolecules is very clear, but often idealized. This is why students of modern biochemistry should at least familiarize themselves with methods of structural analysis during their studies so as to be able to distinguish between what has been measured and what is calculated, what is reality and what fiction, when looking at the structure of the database.
Absolventi kurzu získají teoretické znalosti z oblasti funkční a strukturní biochemie a také praktické dovednosti zahrnující použití různých metodologických postupů a využití mnoha moderních instrumentálních metod při studiu struktury a funkce živých organizmů na molekulární úrovni.
Prerequisites
The minimum requirement for students is basic knowledge of chemistry and biochemistry acquired after completing university education of biological, chemical or biochemical specialization.

Assessment methods and criteria
Written examination, Combined exam

The requirement for successful completion of the course is the active participation of students in lectures and seminars. The final exam will take the form of a written test and a subsequent oral interview with the final evaluation in the form of awarding the mark.
Recommended literature
  • Kensal E. van Holde, W. Curtis Johnson, P. Shing Ho: Principles of physical biochemistry 2nd ed. Pearson Education, Inc. (2006), ISBN 0-13-046427-9.
  • Žídek, L. a kol.: Strukturní biochemie, MU Brno, 2015.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester